定位性兔髂动脉粥样硬化模型的建立

吴志勇 颜光烈 浦晓东等定位性兔髂动脉粥样硬化模型的建立[J].吉林医学,2014(19):7-9.

吴志勇 颜光烈 浦晓东 陈诗泉

福建医科大学省立临床医学院福建省立医院心内科,福建福州,350000

2014

4137-4140

知网,万方

采用高脂喂养与动脉内膜球囊损伤相结合的方法建立定位性兔髂动脉粥样硬化模型。方法：48只新西兰白兔随机分成两组：对照组：基础饲料+假手术（ n＝6）；模型组：饲以1%胆固醇、6%猪油的高脂饲料8周，进食高脂饲料后1周内皮剥脱（n＝42）。酶学法测定血脂全套。8周时进行髂动脉造影，处死对照组及部分模型组（n＝6）行光镜检查，部分行透射电镜及扫描电镜。结果：模型组中所有髂动脉都有不同程度的狭窄，大约15%~100%，平均61.47%；对照组未见明显狭窄。血清TG，TC，LDL-C在模型组中均显著增高（ P﹤0.01），而血清HDL-C在模型组均显著减少。组织病理学检查显示内皮细胞再生，形态不规则、较大，排列紊乱，以及明显内膜增厚，粥样斑块形成，其内主要含泡沫细胞、平滑肌细胞及纤维结缔组织增生。结论：高脂饮食及内皮损伤可在短期内成功复制定位性动脉粥样硬化模型，为动脉粥样硬化及再狭窄的研究提供实验基础。 Objective To develop the focal atherosclerotic model induced by feeding high-fat diet combined with intimal balloon injury in rabbit iliac artery. Method New Zealand White rabbits( n=48 )were randomly divided into two groups:control group:a standard diet and sham operation(n=6);model group:an atherogenic diet with 1% cholesterol and 6% pig oil for 8 weeks and endothelial dedutation 1 week after atherogenic diet(n=42). Before and after 8 weeks of dietary intervention,blood samples were collected for enzymatic measure-ments of serum triglyceride( TG),total cholesterol( TC),low-density lipoprotein cholestrol( LDL-C)and high-density lipoprotein cholestrol( HDL-C)levels. At the end of the dietary intervention,iliac artery quantitative angiography was made and iliac arteries in con-trol and partial model group(n=6)were examined by light microscopy,some of which by scanning(SEM)and transmission electron mi-croscopy( TEM). Results All iliac arteries had various degrees of stenosis ranging from 15% to 100% with a average of 61. 47% in model group,but not in control group. Serum TG,TC and LDL-C were signficantly increased( P﹤0. 01)but serum HDL-C was remarkably de-creased in model group compared with control group,respectively. Histopathologic examination showed reendothelialization with large irregu-larly shaped and disoriented endothelial cells and marked intimal thicking with typical atherosclerotic plaque containing foam cells,smooth mucle cells and connective tissue elements. Conclusion Focal atherosclerotic model in rabbit iliac artery is successfully replicated by com-bining an atherogenic diet with endothelial dedutation within a short period of time,It builds up an experimental foundation in the researchs on atherosclerosis and restenosis.