大蒜素对气道平滑肌细胞α一平滑肌肌动蛋自表达的抑制作用及其机制研究

魏晓阳.大蒜素对气道平滑肌细胞α一平滑肌肌动蛋自表达的抑制作用及其机制研究[J].中华危重病急救医学,2013,25(3),164-166

大蒜素，支气管哮喘，α一平滑肌肌动蛋白，Small蛋白

魏晓阳

北京，解放军总医院第一附属医院呼吸科

2013

164-166

知网

目的观察大蒜素对气道平滑肌细胞(ASMC)。一平滑肌肌动蛋白(a-S M A)表达的影响，以评价大蒜素对气道重塑的抑制作用，并探讨其相应的作用机制。方法用。,2.5,5.0,10.0 p,mol/L的大蒜素处理人ASMC细胞48 b后提取细胞总RNA和蛋自。荧光定量聚合酶链反应(PC R)检测细胞。-SM A mRNA表达，蛋白质免疫印迹法(Western blotting)检测细胞。-SM A和磷酸化Smadl ( p-Small 1)的蛋白表达。结果随着大蒜素处理ASMC细胞浓度的提高(0,2.5,5.0,10.0林mol/T.)，细胞。-SVLA mRNA表达呈进行性下降(分别为0.543士0.121,0.354士0.072 , 0.223士0.058,0.191士0.034，各剂量间两两比较差异有统计学意义(均尸< 0.05 ) ; a-SM A和p-Small 1的蛋白表达也呈进行性下降〔a-SMA蛋白(灰度值比值)分别为。.96士0.02 , 0.72士。.16, 0.54士0.11,031士0.14 , p-Smadl蛋自(灰度值比值)分别为0.94士0.03 ,0.76士0.13 , 0.62士0.11 ,0.43士0.12，各剂量间两两比较差异有统计学意义(均P< 0.05 )。结论大蒜素可以剂量依赖性地下调AS}TC细胞a-SMA的mRN.A和蛋白表达，抑制，nad蛋自的磷酸化，阻止转化生民因子书(TGF-团和Rho激酶信号通路。 　　To insestigate the effectf)fallicin on the expression of a一*month muscle actin(-SMA) in airway smooth muscle cells (ASMC)，and to evaluate the mechanism of allicin。，:inhibition of airwayremodeling. Methods The human ASMCs were treated for 48 hours wth 0. 2.5. 5.0, and 10.0 p,mol/L allicin,respectively, and the total RNA and protein of the cells were col土。cted. The mRNA expression of a -SMA wasdetermined by real-time polymerise chain reaction (PCR)analysis. The protein expression of。-SMA andphosphorylation Smadl (p-Smadl)were assessed by V}estern blotting analysis. Results After the treatment withallicin for 48 hours in a dose of 0, 2.5 , 5.0, and 10.0 p mol/L respectively, the mRNA expression of a -SM A wasdown-regulated ( 0.543士0.121，0.354士().072，0.223士0.058.and 0.191士0.034, respectisel})，wth statisticalhsignificant difference among groups ( all P < 0.05)，and the protein expression of。-S11A and p-Smadl was alsogradually down-regulated、。a close-dependent manner〔-SMA protein } grad value ratio):0.96士0.02，0.72士0.16.0.54士0.11，and 0.31士0.14, respectively; p-Smadl protein(ad value ratio ):0.94士0.03，0.76士0.13，0.62士0.11，and 0.43士0.12, respectively)，with statistically significant difference among gTOUps(all P