补肾助孕汤对小鼠生殖能力影响的实验研究

李小妮，李雅璇，周吉海等.补肾助孕汤对小鼠生殖能力影响的实验研究[J].中国中西医结合杂志.2013, 33(3):365-369

补肾助孕汤;生殖能力;白血病抑制因子;表皮生长因子;降钙素

李小妮，李雅璇，周吉海，谭季春

中国医科大学附属盛京医院辅助生殖中心 沈阳110004

2013

365-369

知网,万方

目的 探讨中药补肾助孕汤对小鼠生殖能力的影响.方法 60只昆明雌性小鼠随机分为实验组与对照组.实验组每天2次经鼠胃管灌注补肾助孕汤,对照组同时给予生理盐水灌胃,分别于给药10、20天处死小鼠,每次每组15只,放射免疫法检测血清雌二醇和孕酮水平；HE染色,行子宫、卵巢组织形态学观察；免疫组化法测定子宫内膜白血病抑制因子(leukaemia inhibitory factor,LIF)、表皮生长因子(epidermal growth factor,EGF)和降钙素(calcitionin,CT)表达.结果 给药10、20天,实验组小鼠子宫、卵巢重量,血清雌二醇、孕酮水平以及子宫内膜LIF、EGF、CT表达均较对照组同期增加,差异有统计学意义(P ＜0.05,P＜0.01),且以给药20天作用为优(P＜0.05).组织学检查显示,实验组小鼠卵巢各级卵泡数目增加,子宫内膜腺体数目增多,子宫内膜增生,复层排列,基质细胞增多,以给药20天最明显.结论 补肾助孕汤可以促进小鼠生殖器官的发育,促进雌二醇、孕酮分泌及卵泡生长；增加子宫内膜的容受性,从而增强其生殖能力. Objective To study the effects of Bushen Zhuyun Decoction(B ZD)on the reproduc- tive capacity of mice. Methods Totally 60 female Kunming mice were randomly divided into the experi- mental group and the control group. BZD was perfused by gastric tube to mice in the experimental group， twice daily.Meanwhile，mice in the control group were administered with normal saline by gastrogavage. Mice were sacrificed on the 10th day and the 20th day of medication respectively，15 mice each time in each group. The serum levels of estradiol and progesterone were detected by radioimmunoassay.The morphological changes of uterus and ovary were observed using HE staining.The expressions of leukae- mia inhibitory factor(LIF)，epidermal growth factor(EGF)，and calcitionin(CT) were determined using immunohistochemical assay.Results Compared with the control group at the same time point，the weights of uterus and ovary increased，the serum levels of estradiol and progestogen increased，the ex- pressions of LIF，CT, and EGF increased in the experimental group on the 10th day and the 20th day of medication (P