正交试验法优选防风感冒颗粒制备工艺

刘效栓,李喜香,焦正花，等、正交试验法优选防风感冒颗粒制备工艺.[J].中国中医药信息杂志,2011,18(1).64-66

正交试验法;防风感冒颗粒;提取工艺

刘效栓,李喜香,焦正花,李季文,王炯,梁海宁

甘肃省中医院,甘肃 兰州 730050

2011

64-66

知网,万方

目的 优选防风感冒颗粒的提取纯化工艺。方法 分别利用正交试验法,考察加水量、提取次数、提取时间对提取工艺的影响,浓缩液比重、醇沉浓度对醇沉工艺的影响,每个因素设计3个水平,以黄芩苷含量和干浸膏为考察指标。用高效液相色谱法测定黄芩苷含量,烘干法测定干浸膏得率。结果 最佳提取工艺为：加8倍量水,煎煮3次,每次2 h;最佳醇沉工艺为：醇沉浓度60%,浓缩液比重1.05 g/mL。结论 正交试验法筛选防风感冒颗粒的提取纯化工艺稳定、可行、简便,为大生产提供了试验依据。 Objective To optimize the extraction and purification technology of Fangfeng Ganmao Granules. Methods The extraction process was optimized by orthogonal test, with water addition, extraction times and time used for extraction as factors of investigation. In refined process test, alcohol precipitation concentration and the relative density of extract were studied. Each factor had three levels. The baicalin and the yield of dry extract were used as the evaluation indexes. The baicalin was determined by HPLC, and dry extract rates were determined by drying method. Results The best extracting technology condition was as follows：the amount of the water was 8 times of the medicinal materials, the decoction duration was 2 h and for 3 times. The optimum purification process was：alcohol precipitation concentration was 60%, relative density of extract was 1.05 g/mL. Conclusion The optimized process of Fangfeng Ganmao Granules is stable, feasible and convenient. It provides experiment basis for mass production.