慢性乙型肝炎患者肝组织低氧诱导因子1α的表达变化

丁体龙,范秀梅,王勇等.慢性乙型肝炎患者肝组织低氧诱导因子1α的表达变化[J].临床肝胆病杂志, 2013, 29(2).124-127

细胞低氧 知识脉络 肝炎,乙型,慢性 知识脉络 微循环 知识脉络

丁体龙[1],范秀梅[2],王勇[1],黄奕江[1],马勇[1]

解放军第123医院南京军区肝病中心,安徽蚌埠,233015；蚌埠医学院临床诊断学教研室,安徽蚌埠,2

2013

124-127

知网,万方

目的 研究肝组织中低氧诱导因子(HIF)-1α的表达与慢性乙型肝炎(CHB)患者肝脏微循环障碍的关系.方法 对81例CHB患者和5例正常人的肝标本进行免疫组织化学染色,并用透射电镜观察肝组织超微结构的变化.结果 HIF-1α在肝组织中的表达强度和范围与CHB患者肝脏微循环障碍程度密切相关,电镜示肝窦腔内红细胞聚集、狄氏腔中胶原纤维沉积及肝窦内皮细胞表面有基底膜形成.结论 HIF-1α通过徼活血管增生,最终导致血管纤维化和肝窦毛细血管化,加重肝脏微循环障碍. Abstract W rbances :Objective To study the relationship between expression of hvpoxia in the liver microcirculatory system in patients with chronic 一inducible factor一1 alpha(HIF一1a} in liver tissr。and dis- B(CHB).Methods Liver biopsy samples were taken from 81 patrents of each with CHB and five healthy controls. HIF一1 a expression was hepatitis detected in each sample by immunohistochemistry sample was observed by transmission electron microscope(TEM).Multiple comparisons were carried out by the and the ultrastructure Kruskal一}C alli} rank teat HTF' usme 1 一1 (Y a completely expression m randomized design different liver cell and pairwise multiple comparisons were carried out by the Nemenyi test. Results Distribution of topes was closely related to the degree of liver microcirculatory disturbance in patients TEM showed that CHB liver samples had erythrocytes aggregated in the sinusoids, collagen fibers deposited in the Disse space kith CHB. and a basal membrane illarization formed on the surface of the sinusoidal endothelial cells. Conclusion HIF一ltv may induce blond vessel fibrosis and sinusoidal cap- in the liver