足细胞标志蛋白检测在糖尿病肾病中的临床应用价值

发表时间：2014-02-08 浏览次数：910次

引用：

柴晓文,张雪宁,李励.足细胞标志蛋白检测在糖尿病肾病中的临床应用价值[J].中国医师进修杂志,2013,36(10):14-16.

关键词：

糖尿病肾病足细胞足细胞标志蛋白

作者：

柴晓文张雪宁李励

作者单位：

天津市大港油田总医院检验科，天津市大港油田港东医院检验科,天津市残疾人康复服务指导中心

出版年份：

2013

期刊页数：

14-16.

收录者：

万方

摘要：

目的检测糖尿病肾病(DN)患者足细胞标志蛋白(PCX)表达的变化,并探讨其相关的临床意义. 方法选择DN患者68例(DN组),按尿白蛋白与肌酐比值分为三个亚组:正常蛋白尿组(＜ 30mg/g,A组)、微量白蛋白尿组(≥30 mg/g而＜300 mg/g,B组)、大量白蛋白尿组(≥300 mg/g,C组).另选择健康体检者20例作为对照组,酶联免疫吸附试验法检测尿沉渣中PCX表达水平. 结果 DN组患者尿沉渣中PCX、血肌酐、血尿素氮的表达水平显著高于对照组(27.44±6.87) μg/L比(3.52±1.13) μg,/L、(154.82±11.54) μmol/L比(82.56±5.71)μmol/L、(8.79±0.58) mmol/L比(4.23±0.34) mmol/L,P＜0.01]；A、B、C组PCX的表达水平分别为(18.04±4.12)、(23.58±5.43)、(32.18±6.05)μg/L,差异均有统计学意义(P＜0.05)；A、B、C组血肌酐、血尿素氮分另为 (138.54±8.66)、(142.16±11.74)、(165.13±12.85) μmol/L,(8.48±0.43)、(8.56±0.61)、(10.04±1.17) mmol/L,血肌酐、血尿素氮在A组和B组比较差异无统计学意义(P＞0.05),但在A组和C组、B组和C组比较差异有统计学意义(P＜0.05).结论 DN患者尿沉渣中存在PCX的高表达,检测PCX有利于DN患者肾损害的早期诊断和病变程度的评估. Objective To investigate the expression ofpodocalyxin(PCX) in patients with diabetic nephropathy (DN) and its clinical significance.Methods A total of 68 patients with DN were enrolled as DN group.Another 20 healthy person were selected as control group.The patients with DN were divided into three sub-groups:normal albuminuria group(＜ 30 mg/g,group A),microalbuminuria group(≥30 mg/g but ＜ 300 mg/g,group B) and macroalbuminuria group (≥ 300 mg/g,group C).The level of urinary PCX was measured with enzyme-linked immunosorbent assay.Results The level of urinary PCX,serum creatinine and blood urea nitrogen in DN group was higher than that in control group [(27.44 + 6.87) μ g/L vs.(3.52 +1.13) μ g/L,(154.82 ± 11.54) μ mol/L vs.(82.56 ± 5.71) μ mol/L,(8.79 ± 0.58) mmol/L vs.(4.23 ± 0.34)mmol/L,P ＜0.01].The level of urinary PCX in group A,B,C was (18.04 ±4.12),(23.58 ±5.43),(32.18 ± 6.05) μ g/L,and there was significant difference among three groups (P ＜ 0.05).The level of serum creatinine and blood urea nitrogen was (138.54 + 8.66),(142.16 ± 11.74),(165.13 ± 12.85) μ mol/L and (8.48 ± 0.43),(8.56 ± 0.61),(10.04 + 1.17) mmol/L in group A,B,C,there was no significant difference between group A and group B(P＞ 0.05),but there was significant difference between group A and group C,or between group B and group C (P ＜ 0.05).Conclusion The high-level expression of urinary PCX is found in DN patients,which maybe play a key role in the early diagnosis and assessment of the progression of DN.

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