牙髓卟啉单胞菌内毒素诱导成骨细胞表达炎症因子的信号通路研究
发表时间：2014-05-19 浏览次数：829次
杨谛，仇丽鸿，李任等、牙髓卟啉单胞菌内毒素诱导成骨细胞表达炎症因子的信号通路研究、[J].华西口腔医学杂志,2010,7(2):
牙髓卟啉单胞菌； 内毒素； 成骨细胞； 细胞外信号调节激酶1/2； p38丝裂原活化蛋白激酶
杨谛1 仇丽鸿1 李任1 李子木1 李琛2
1.中国医科大学口腔医院 牙体牙髓科；2.中国医科大学口腔医学院 中心实验室，辽宁 沈阳 11000
2010
知网
目的 检测细胞外信号调节激酶（ERK）1/2和p38丝裂原活化蛋白激酶抑制剂对牙髓卟啉单胞菌内毒素（LPS）诱导成骨细胞白细胞介素（IL）-1β mRNA和IL-6 mRNA的影响，探讨根尖周病变牙槽骨吸收的可能病理机制。方法 成骨细胞MG-63经PD98059和SB203580预处理1 h后，加入牙髓卟啉单胞菌LPS作用6 h，应用逆转录聚合酶链反应（RT-PCR）检测IL-1β mRNA和IL-6 mRNA的表达水平。结果 PD98059预处理后，牙髓卟啉单胞菌LPS诱导MG-63表达IL-1β mRNA的水平下降。SB203580预处理后，牙髓卟啉单胞菌LPS诱导MG-63表达IL-1β mRNA和IL-6 mRNA水平均下降。结论 牙髓卟啉单胞菌LPS诱导MG-63细胞表达IL-1β mRNA依赖ERK1/2和p38MAPK信号转导通路，表达IL-6 mRNA依赖p38MAPK信号转导通路。 Objective To quantify the interleukin（IL）-1β mRNA and IL-6 mRNA expression induced by lipo-polysaccharides（LPS） extracted from Porphyromonas endodontalis（P.endodontalis） in osteoblasts, and to relate P. endodontalis LPS to the bone resorptive pathogenesis in the lesions of chronic apical periodontitis. Methods MG63 cells was pretreated with PD98059 or SB203580 for 1 h and then treated with P.endodontalis LPS for 6 h. The expression of IL-1β mRNA and IL-6 mRNA were detected by reverse transcription polymerase chain reaction（RT-PCR） technique. Results The production of IL-1β mRNA induced by P.endodontalis LPS decreased in osteoblasts pretreated with PD98059. Both of the production of IL-1β mRNA and IL-6 mRNA induced by P.endodontalis LPS decreased in osteoblasts pretreated with SB203580. Conclusion The synthesis of IL-1β mRNA stimulated by P.endodontalis LPS in MG63 probably occur via extracellular signal-regulated kinase（ERK） 1/2 and p38 mitogen activated protein kinase（MAPK） signal transduction system. The synthesis of IL-6 mRNA stimulated by P.endodontalis LPS in MG63 probably occur via p38MAPK signal transduction system.
