新生儿人巨细胞病毒先天性感染危险因素筛查
发表时间：2014-05-16 浏览次数：770次
汪晓婷， 陈灵芝， 陈敬贤等、新生儿人巨细胞病毒先天性感染危险因素筛查、[J].中华疾病控制杂志,2012,16(6): 483-185
婴儿，新生; 巨细胞病毒; 危险因素
作者：汪晓婷1， 陈灵芝1， 陈敬贤2， 王明丽1
1安徽医科大学微生物学教研室， 安徽 合肥 230032 2美国哥伦比亚大学病理与细胞生物学系， 纽
2012
483-185
知网,万方
目的 通过检测新生儿脐带血中人巨细胞病毒(human cytomegalovirus， HCMV)IgG抗体和gB基因，了解新生儿HCMV感染情况，并结合临床资料分析产妇年龄、Ⅰ型单纯疱疹病毒(herpes simplex virus， HSV-Ⅰ)IgG抗体阳性、孕周与HCMV先天性感染的相关性。 方法 收集2010年11月～2011年6月期间，某市妇幼保健院就诊的健康产妇所娩新生儿脐带血，共178例。采用ELISA法检测脐带血血清是否存在HCMV IgG和HSV-Ⅰ IgG抗体，巢式PCR检测HCMV gB基因。 结果 产妇HCMV IgG阳性率为99.44%(177/178);产妇HSV-Ⅰ IgG阳性率为83.71%(149/178);新生儿脐血HCMV gB阳性率为32.02%(57/178);产妇年龄和HSV-Ⅰ IgG抗体阳性不是发生HCMV先天性传播的危险因素。 结论 针对早产和过期产儿，应该加强其HCMV先天性感染的早期诊断。 Objective To screen the congenital human cytomegalovirus (HCMV) infection in newborns. To detect whether there are relationships between the maternal age， HSV-Ⅰ IgG antibodies， premature and expired births with the congenital human cytomegalovirus infection. Methods To collect the neonatal cord blood from the Maternity and Child Health Hospital from November 2010 to June 2011. Serum HCMV-IgG and HSV-Ⅰ IgG were screened in 178 cord blood by enzyme-linked immunosorbant assay (ELISA)， and among them， HCMV DNA were detected by nest-polymerase chain reaction (nest-PCR). Results The positive rate of HCMV-IgG was 99.44%(177/178); The rate of congenital HCMV infection was 32.02%(57/178). The positive rate of HSV-Ⅰ IgG was 83.71%(149/178); Maternal age and HSV-Ⅰ IgG antibodies were not risk factors for HCMV congenital transmission. Conclusions Early diagnosis should be strengthened for premature and expired births children about HCMV congenital infection.
