脂多糖诱导气道上皮细胞过氧化物氧化还原酶1的表达

发表时间：2014-06-09 浏览次数：1385次

引用：

刘伟婷，毛璞，刘晓青，何为群，莫红缨，黎毅敏.脂多糖诱导气道上皮细胞过氧化物氧化还原酶1的表达[J].中华危重病急救医学,2013,25(3),136-139

关键词：

作者：

刘伟婷，毛璞，刘晓青，何为群，莫红缨，黎毅敏

作者单位：

广州医学院第一附属医院重症医学科

出版年份：

2013

期刊页数：

136-139

收录者：

知网

摘要：

　　目的探讨脂多糖汀.PS)作用于气道上皮细胞后对过氧化物氧化还原酶1(prdxl)表达的影响。方法培养正常人气道l二皮细胞株BEAS-2B，以0,1 ,10 mg/L LPS作用于气道上皮细胞12h和24 h后，用逆转录一聚合酶链反应(RT-PCR)检测prdxl mRNA表达;以0,0.1,0.5,1,5,10 mg/L LPS作用于气道上皮细胞121，后，用蛋白质免疫印迹试验(Western blotting)检测prdxl蛋白表达。结果RT-PCR结果显示:LPS作用于细胞12h内，随着LPS作用剂量的增加，prdxl mRNA表达增高，10 mg/L LPS作用12 h prdxl mRNA表达较对照组显著增加(2.014士0.197比0.644士0.178 , P< 0.05;但随着LPS作用时间的延长，24 h时prdxl mRNA表达有所回落。Western blotting结果显示，随着LPS作用剂量的增加，prdxl蛋白表达逐渐增高，5 mg/L LPS作用72h时prdxl蛋自显著高于对照组((1.069士0.175比0.328士0.010 , P< 0.05 ) ,10 mg/h LPS作用12h时维持在高水平恤984士0.220)结论10 mg/L的LPS作用于BEAS-2B细胞12h后可诱导prdxl的基因和蛋白表达增加。　　To investigate the effect of lipopolysaccharide (LPS) nn expression of peroxiredoxin 1( prdxl)in airway epithelial cells. Methods The airway epithelium cell line BEAS-2B was cultivated, and the cellswere stimulated with 0, 1，and 10 mg/L of LPS for 12 hours and 24 hou:一*.and then were harvested for prdxl expressiondetection. The mRNA xpression of prdxl was detected by reverse transcriptio，卜pohmerase chain reaction (RT-PCR). 　　The aimay epithelium cells were stimulated with 0, 0.1，0.5，1，5，and 10 mg/L of LPS for 12 hours, and werecollected for determination of prdx 1 protein expression by }~ astern blotting. Results RT-PCB results showed that theprdxl mRNA expression was significantly increased within 12 hours of stimulation with elevation of the dosage of LPS. 　　The prdxl mRNA expression at 12 hours of stimulation by 10 mg/L I_PS was significantly hi4her than that in control group( 2.014士0.197 vs. 0.644士0.178，P < 0.05).However, with prolongation of LPS stimulation time, the prdxl mRNAexpression at 24 hours was slightly declined.Wastern blotting results showed that the prdxl protein expression wasgradually increased with elevation of dosage of LPS. The prdxl protein expression at 12 hours of stimulation withSmL LPS was significantly higher than that in control group(1.069士0.175 vs. 0.328士0.010, P

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