高效液相色谱法测定百花定喘片中橙皮苷的含量
发表时间:2014-06-03 浏览次数:1064次
秦香芹,任敬超.高效液相色谱法测定百花定喘片中橙皮苷的含量[J].黑龙江医药,2012,25(2).194-195
高效液相色谱法;百花定喘片;橙皮苷
秦香芹,任敬超
哈尔滨瀚邦医疗科技有限公司
2012
194-195
知网
目的:建立高效液相色谱法测定百花定喘片中橙皮苷的含量。方法:以十八烷基硅烷键合硅胶为填充剂,甲醇-醋酸-水(35:4:61)为流动相,检测波长283nm。结果:平均回收率为98.26%,RSD为0.89%。结论:该方法简便、准确。 Objective:To establish a method of hesperidin for baihuadingchuan tablets by high-performance liquid chromatography.Methods:The method uses a C18 column,methanol- acetic acid-water(35:4:61)as the mobile phase.The detection wavelength was set at 283nm.Results:The average recovery was 98.26%,RSD was 0.89%.Conclusion:The method is simple and accurate。