高效液相色谱法测定肌氨肽苷注射液中次黄嘌呤的含量
发表时间:2014-06-03 浏览次数:1114次
李明兰,秦香芹.高效液相色谱法测定肌氨肽苷注射液中次黄嘌呤的含量[J].黑龙江医药,2012,25(2).192-193
高效液相色谱法;肌氨肽苷注射液;次黄嘌呤
李明兰1,秦香芹2
1.哈尔滨圣泰制药股份有限公司;2.哈尔滨瀚邦医疗科技有限公司
2012
192-193
知网
目的:建立高效液相色谱法测定肌氨肽苷注射液中次黄嘌呤的含量。方法:以十八烷基硅烷键合硅胶为填充剂,磷酸二氢钾(0.01mol/L)-甲醇(100:0.2)为流动相,检测波长260nm。结果:平均回收率为100.56%,RSD=1.57%。结论:该方法简便、准确。 Objective:To establish a method of hypoxanthine for Jiantaigan injection by high-performance liquid chromatography.Methods:The method uses a C18 column,potassium dihydrogen phosphate(0.01mol/L)- methanol(100:0.2)as the mobile phase.The detection wavelength was set at 260nm.Results:The average recovery was 100.56%,RSD was 1.57%.Conclusion:The method is simple and accurate.