终末期肾病维持性血透患者树突状细胞分化成熟能力的研究

发表时间：2014-06-18 浏览次数：999次

引用：

吴俊逸，张薇.终末期肾病维持性血透患者树突状细胞分化成熟能力的研究[J].2013, 33(3):290-293

关键词：

终末期肾病;维持性血液透析;树突状细胞;表而分子;细胞因子

作者：

吴俊逸，张薇

作者单位：

上海交通大学医学院附属第九人民医院肾脏内科,上海,200011

出版年份：

2013

期刊页数：

290-293

收录者：

知网,万方

摘要：

目的研究终末期肾病(ESRD)患者外周血中树突状细胞(DC)分化成熟能力的变化,探讨ESRD患者免疫功能低下的可能机制.方法采集接受维持性血液透析治疗的ESRD患者(ESRD组,n=20)外周血,利用粒-巨噬细胞集落刺激因子(GM-CSF)、白介素4(IL-4)和脂多糖(LPS)联合培养体系体外诱导培养DC,经流式细胞仪检测DC表面分子CD40、CD80、CD86 、CD83、CCR7和HLA-DR的表达,采用酶联免疫吸附法(ELASA)测定DC细胞培养上清液中细胞因子IL-12的含量.设立正常对照组(n=20).结果与正常对照组比较,ESRD组DC表面分子CD40 、CD80 、CD86、CD83、CCR7和HLA-DR的表达率显著降低,组间比较差异均有统计学意义(P ＜0.05和P＜0.01)；同时,ESRD组DC培养上清液中IL-12的含量显著高于正常对照组(P＜0.01).结论 ESRD患者外周血单核细胞源性的DC发育不成熟,其趋化迁移功能以及外源性抗原递呈能力下降,免疫激活能力降低,可能导致T细胞功能缺陷,从而最终诱导机体特异性的免疫耐受. Objective 1"o investigate the changes of capacity of differentiation and maturation of dendritic cells(DC) in peripheral blood in patients with end一、tage renal disease(ESRD) undergoing maintenance hemodialvsis, and explore the possible mechanism of impaired immune responses of patients with ESIiD. Methods The peripheral blood samples of patients with ESRD undergoing maintenance hemodmlysis(ESRD group，，=20) we:二collected. and in vitro culture with granulocyte一，nacrophage colony一*timulating factor(G IVl一CSF)，interleuki:卜4(IL一4)and lipopolysaccharide ( LPS〕was conducted.The expression of CD40, CD80, CD86, CD83, CCR7 and HLA一DR on the surface of DC was detected by flow evtornetry. and the level of interleukin一12(1L一12)in the culture supernatant of DC was determined by enzyme一!inked immunosorhent assay(ELISA).Besides, normal control group was established(;:=20).Results The expression of CD40, CD80, CD86, CD83，CCR7 and HLA-DR on the surface of DC in ESRD group was significantly lower than that in normal control group(P

阅读全文