成骨细胞培养微环境对Hela细胞STAT3的转录表达及细胞增殖的调控作用

发表时间：2014-01-21 浏览次数：517次

引用：

王森，陈荏槐，陈泓臻等.成骨细胞培养微环境对Hela细胞STAT3的转录表达及细胞增殖的调控作用[J].吉林医学,2013,34(36):7574-7575.

关键词：

宫颈癌；微环境；成骨细胞

作者：

王森，陈荏槐，陈泓臻，姜恩平，康海仙，赵毅，姚运红，胡新荣，朱伟

作者单位：

广东医学院肿瘤研究所，广东东莞 523808

出版年份：

2013

期刊页数：

7575-7576

收录者：

万方

摘要：

目的：探讨成骨细胞微环境（OBM）对Hela细胞STAT3转录表达及细胞增殖的影响。方法：分离培养大鼠成骨细胞，取其培养液与DMEM血清培养基按照1:1比例混合，培养Hela细胞。分为对照组与实验组（添加成骨细胞培养液）。采用Real time PCR检测 STAT3的mRNA水平，CCK-8法检测Hela细胞增殖。结果：成功分离成骨细胞，并取其培养液作用于宫颈癌Hela细胞。与对照组相比，在24 h、48 h、72 h，实验组STAT3 mRNA水平分别升高约28.2%、37.5%、32.1%，实验组细胞增殖能力分别升高约11.7%、29.5%、 24.6%。结论：OBM可上调宫颈癌Hela细胞中STAT3的转录表达并促进细胞增殖。 Objective To investigate the osteogenic microenvironment(OBM) on the proliferation of Hela cell STAT3 expression and cell. Methods Rat osteoblasts were cultured,and the medium and DMEM serum medium are mixed according to the proportion of 1:1,the cultured Hela cells.Divided into the control group and the experimental group(added osteoblast cell culture fluid).Using Real time PCR to detect STAT3 mRNAlevels,Hela cell proliferation was detected by CCK-8.Results The successful isolation of osteoblasts,and the medium role in cervical cancer Hela cells.Compared with the control group, at 24 h,48 h,72 h,experimental group STAT3 mRNA levels were increased about 28.2%,37.5%,32.1%,the experimental group cells were increased about 11.7%,29.5%,24.6%.Conclusion OBM transcriptional upregulation of STAT3 expression in cervical cancer Hela cells and promote cell proliferation.

阅读全文