不同冲洗剂冲洗根管杀菌效果的体外观察

发表时间：2014-04-08 浏览次数：981次

引用：

高爽，李朋莲，李振玲，等.不同冲洗剂冲洗根管杀菌效果的体外观察[J].吉林医学,2014,35(10):2030-2032.

关键词：

臭氧水溶液;粪肠球菌;杀菌能力

作者：

高爽，李朋莲，李振玲，刘佳柏，卢春英，张志民

作者单位：

吉林大学口腔医院，吉林大学口腔医学院，吉林长春130021

出版年份：

2014

期刊页数：

2030-2032.

收录者：

知网,万方

摘要：

目的：比较次氯酸钠溶液与不同浓度臭氧水溶液的杀菌效果，为臭氧水溶液应用于临床感染根管的冲洗提供微生物学基础。方法：将根尖发育完全的单直根管离体牙经一系列标准化处理后，建立离体牙的E.faecalis体外感染模型，采用Mtwo机用镍钛器械和冠向下法预备根管，稀释平板计数法分别测定各实验组冲洗前、冲洗后24 h和7 d时E.faecalis的CFUml-1。Mann-Whitney U检验法进行数据统计分析。结果：冲洗前各组根管内细菌数量的差异无统计学意义(P>0.05)。经冲洗剂处理后，培养24 h各实验组对根管内E.faecalis的杀灭率均为100%，7 d时均有少量E.faecalis被检出，但三组之间无统计学差异(P>0.05)。结论：5.25%NaCl溶液、3.994 mg/L臭氧水溶液和1.339 mg/L臭氧水溶液对根管内的E.faecalis均有瞬时杀灭作用，但却不能完全将其清除，只能减少它的数量，使其在一段时间内处于抑制状态;臭氧水溶液对根管内E.faecalis的杀灭能力与其浓度呈正相关。 Objective To evaluate the microbicidal efficacy between the sodium hypochlorite and the ozonated water of different concentration，provide the microbiological foundation for the application of ozonated water as a root canal irrigating agent in clinical.Method Standardize the extracted human tooth.After establishing the E.faecalis infected model，preparing the roots through the step-down technique with sterile Mtwo machine nickel-titanium instruments.Using the plate count assay the CFU/ml of E.faecalis before andafter irrigation.Data was analyzed by Mann-Whitney tests.Results Before irrigation，there was no statistic differences of the CFU amongthree groups(P>0.05).After irrigation，the 24-hour killing ratio of the three irrigating agents on E.faecalis in root canals were all 100%.But after 7 days，a small amount of E.faecalis had been detected，The difference was not statistically significant among the three groups(P>0.05).Conclusion The E.faecalis in root canals can be killed instantaneously by the three irrigating agents，but can not be removedoff，can only reduce in its quantity and inhibited during a period of time;the concentration and the microbicidal efficacy of ozonated waterare positively correlated.

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