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《病理学与病理生理学》

三种不同方法检测HLA-B27的比较

发表时间:2014-09-05  浏览次数:1775次

引 用:

徐炳燕,黎四平,彭琪等.三种不同方法检测HLA-B27的比较[J].吉林医学,2014,(22):4845-4846.

关 键 词:

强直性脊柱炎;人类白细胞抗原B27;流式细胞术

作者:

徐炳燕,黎四平,彭琪,梁丽芸,叶俊琴

作者单位:

(广东医学院附属石龙博爱医院检验科,广东东莞523325)

出版年份:

2014

期刊页数:

4845-4846

收录者:

知网,万方

摘要:

目的:比较强直性脊柱炎患者HLA-B27常用的三种检测方法的特点,探讨不同检测方法之间差异及各自优缺点,为临床检验提供参考。方法:采用ELISA法、RT-PCR法和流式细胞法,对120例强直性脊柱炎患者和100例正常对照人群进行HLA-B27检测,并对结果进行分析。结果:ELISA法、RT-PCR法和流式细胞法三种检测方法的敏感性、特异性和阳性、阴性预测值分别为83.3%、94.0%、94.3%、82.5%;95.8%、93.0%、94.3%、94.8%;93.3%、95.0%、95.7%、92.2%。结论:三种血清HLA-B27检测方法诊断AS的特异度均较高;PCR法和流式细胞法的敏感度均显著高于ELISA法,其中PCR法略高于流式细胞术。 ObjectiveTo compare three different detection methods of HLA-B27 in patients with ankylosing spondylitis and to explore the differences including their advantages and disadvantages of the three detection methods,to provide reference for clinical diagnosis.MethodThe HLA-B27 of 120 cases of patients with ankylosing spondylitis and 100 healthy control subjects were detected by ELISA,RT-PCR and flow cytometry method,and the results were analyzed.ResultsThe sensitivity,specificity,and positive and negative predictive values by ELISA,RT-PCR and flow cytometry method were 83.3%,94.0%,94.3%,82.5%;95.8%,93.0%,94.3%,94.8%;93.3%,95.0%,95.7%,92.2%.ConclusionThree specificity of serum HLA-B27 by all three methods are high;The sensitivity of PCR method and flow cytometry were significantly higher than ELISA.

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