三种不同方法检测HLA-B27的比较

发表时间：2014-09-05 浏览次数：1741次

引用：

徐炳燕,黎四平,彭琪等.三种不同方法检测HLA-B27的比较[J].吉林医学,2014,(22):4845-4846.

关键词：

强直性脊柱炎；人类白细胞抗原B27；流式细胞术

作者：

徐炳燕，黎四平，彭琪，梁丽芸，叶俊琴

作者单位：

(广东医学院附属石龙博爱医院检验科，广东东莞523325)

出版年份：

2014

期刊页数：

4845-4846

收录者：

知网,万方

摘要：

目的：比较强直性脊柱炎患者HLA-B27常用的三种检测方法的特点，探讨不同检测方法之间差异及各自优缺点，为临床检验提供参考。方法：采用ELISA法、RT-PCR法和流式细胞法，对120例强直性脊柱炎患者和100例正常对照人群进行HLA-B27检测，并对结果进行分析。结果：ELISA法、RT-PCR法和流式细胞法三种检测方法的敏感性、特异性和阳性、阴性预测值分别为83.3%、94.0%、94.3%、82.5%;95.8%、93.0%、94.3%、94.8%;93.3%、95.0%、95.7%、92.2%。结论：三种血清HLA-B27检测方法诊断AS的特异度均较高;PCR法和流式细胞法的敏感度均显著高于ELISA法，其中PCR法略高于流式细胞术。 ObjectiveTo compare three different detection methods of HLA-B27 in patients with ankylosing spondylitis and to explore the differences including their advantages and disadvantages of the three detection methods，to provide reference for clinical diagnosis.MethodThe HLA-B27 of 120 cases of patients with ankylosing spondylitis and 100 healthy control subjects were detected by ELISA，RT-PCR and flow cytometry method，and the results were analyzed.ResultsThe sensitivity，specificity，and positive and negative predictive values by ELISA，RT-PCR and flow cytometry method were 83.3%，94.0%，94.3%，82.5%；95.8%，93.0%，94.3%，94.8%；93.3%，95.0%，95.7%，92.2%.ConclusionThree specificity of serum HLA-B27 by all three methods are high；The sensitivity of PCR method and flow cytometry were significantly higher than ELISA.

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