HPLC测定八珍袋泡茶中芍药苷的含量

发表时间：2014-05-29 浏览次数：1166次

引用：

谢静，杨惠莲，冷静.HPLC测定八珍袋泡茶中芍药苷的含量.[J].中国中医药信息杂志,2011,18(1).62-63

关键词：

八珍袋泡茶;芍药苷;高效液相色谱法;含量测定

作者：

谢静1,杨惠莲1,冷静2

作者单位：

1.重庆市食品药品检验所,重庆 401121;2.重庆市中医院,重庆 400021

出版年份：

2011

期刊页数：

62-63

收录者：

知网,万方

摘要：

目的建立高效液相色谱法测定八珍袋泡茶中芍药苷含量的方法。方法以十八烷基硅烷键合硅胶为填充剂,以乙腈-0.1%磷酸溶液(13∶87)为流动相,检测波长为230 nm。结果芍药苷在0.102 8～1.285 μg范围内呈良好线性,回归方程为A=1 189.9C+7.363 3,r=0.999 9。平均加样回收率为102.0%,RSD=1.4%(n=6)。结论本法简便、准确、专属性强,测定结果重复性好,可以作为八珍袋泡茶中芍药苷的定量分析方法。 Objective To establish a method for determination of paeoniflorin in Bazhen Daipaocha by HPLC. Methods ODS-C18 column (4.6 mm×250 mm, 5 μm) was used, acetonitrile-0.1%phosphoric acid (13∶87) was the mobile phase, detection wavelength was 230 nm. Results The linear range of paeoniflorin was 0.102 8-1.285 μg. The regression equation was A=1 189.9C+7.363 3, r=0.999 9, and the average recovery was 102.0% with RSD of 1.4% (n=6). Conclusion This method was simple, accurate and proper, and the reduplication of the result was good. It can be used to determine paeoniflorin in Bazhen Daipaocha.

阅读全文