Bcl-2在子宫内膜增生及子宫内膜癌的表达及意义
发表时间：2009-06-22 浏览次数：578次
作者:吴韩梅,张淼 作者单位：广东省江门市新会区妇幼保健院，广东江门 529100
【摘要】 目的 探讨bcl2在正常子宫内膜、子宫内膜增生及子宫内膜癌发生和发展中的作用。方法 采用免疫组化SP法，检测20例正常子宫内膜、85例子宫内膜增生和62例子宫内膜癌中的bcl2表达情况。结果 (1) bcl2在正常子宫内膜中阳性表达率为40.0%（8/20），主要表现为增生期腺体胞质的表达。(2) bcl2在子宫内膜增生的总阳性表达率为71.8%(61/85)，与正常子宫内膜相比，差异有统计学意义（P<0.01），其中，bcl2在非典型子宫内膜增生的阳性表达率为87.0%（20/23），在无非典型子宫内膜增生的阳性表达率为66.1%（41/62），两者差异无统计学意义(P>0.05)。(3) bcl2在子宫内膜癌的阳性表达率为50.0%（31/62），与子宫内膜增生相比；差异有统计学意义(P<0.05)； bcl2在不同分化的子宫内膜癌的阳性表达率差异有统计学意义(P<0.05)。结论 bcl2基因可能在子宫内膜增生的发生、发展及子宫内膜癌的早期阶段发挥作用；bcl2表达的监测有助子宫内膜癌癌细胞分化程度的评价。
【关键词】 bcl－2 ；子宫内膜增生 ； 子宫内膜癌
Expression of Bcl2 in proliferative endometrium and endometrial carcinoma
WU Hanmei , ZHANG Miao
(Maternity and Child Healthcare Hospital of Xinhui, Jiangmen 529100,china) Abstract： Objective To study the role of bcl2 in the genesis and development of proliferative endometrium and endometrial carcinoma. Methods The expression of bcl2 were examined with immunohistochemistry technique (sp method) in 62 specimens of endometrial carcinoma, 85 specimens of proliferetive endometrium and 20 specimens of normal endometrium. Results (1) The positive rate of bcl2 protein expression was 40% (8/20) in the normal endometrium, and the expression mainly located in the proliferative phase gland. (2) The total positive expression rate of bcl2 was 71.8% (61/85) in the proliferative endometrium, significantly higher than that in the normal endometrial specimens (P<0.01). The positive rate of bcl2 expression was 87.0% (20/23) in the atypical hyperplasia and 66.1% (41/62) in the nonatypical hyperplasia, with no significant difference (P>0.05) between. (3) The positive rate of bcl2 was 50.0% (31/62) in the endometrial carcinoma, significantly lower than that in the normal endometrium (P<0.05). There was a close relationship between the bcl2 expression and the histological grades of the specimens. Conclusion Low expression of bcl2 gene may contribute to the development of uteroendometrial carcinoma. The expression of Bcl2 may indicate the differentiation grade of the uteroendometrial carcinoma. Key words: bcl2; proliferetive endometrium; endometrial carcinoma 子宫内膜癌是女性生殖系统常见的恶性肿瘤，其发生机制至今尚未明了，现已证实是一个多步骤的复杂过程，有多种癌基因参与。而子宫内膜增生与子宫内膜癌组织学上有密切联系，具有一定的癌变倾向，故被列为癌前病变。子宫内膜增生与癌变的关系一直是病理学界与临床研究的热点。bcl2属于抑制基因，在许多癌症早期有过度表达的报道［1］。本文采用免疫组化方法，研究bcl2在正常子宫内膜、子宫内膜增生、子宫内膜癌中的表达情况，以探讨bcl2 在子宫内膜增生及子宫内膜癌发生、发展及预后的关系，为子宫内膜癌的预后判断及指导临床治疗提供参考。
